Review



phi manipulation treatment  (Selleck Chemicals)


Bioz Verified Symbol Selleck Chemicals is a verified supplier
Bioz Manufacturer Symbol Selleck Chemicals manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 96
      Buy from Supplier

    Structured Review

    Selleck Chemicals phi manipulation treatment
    (A) Representative images of MDCK cells expressing a TOPdGFP reporter plasmid shown according to scale with cell membranes visualized with Cell Mask (magenta). Scale bar = 20µm. (B) Quantification of single-cell reporter intensities after 24 hours of pHi <t>manipulation</t> or WNT3a treatment (40 ng/mL). All cells in each field of view were quantified and were plotted if the average intensity was two-fold higher than the average of mock-transfected cells. Individual cells are shown as block dots with median and interquartile ranges shown in red lines and bars, respectively. LOW, n=103, N=5; CRL, n=181, N=7; HIGH, n=147, N=5; WNT3a, n=103, N=3. Statistical significance in (B) was determined using Kruskal-Wallis test with Dunn’s correction for multiple comparisons. *P<0.05.
    Phi Manipulation Treatment, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 96/100, based on 2826 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phi+manipulation+treatment/bio_rxiv__2024__03__22__586349-175-9-16?v=Selleck+Chemicals
    Average 96 stars, based on 2826 article reviews
    phi manipulation treatment - by Bioz Stars, 2026-08
    96/100 stars

    Images

    1) Product Images from "Intracellular pH regulates β-catenin with low pHi increasing adhesion and signaling functions"

    Article Title: Intracellular pH regulates β-catenin with low pHi increasing adhesion and signaling functions

    Journal: bioRxiv

    doi: 10.1101/2024.03.22.586349

    (A) Representative images of MDCK cells expressing a TOPdGFP reporter plasmid shown according to scale with cell membranes visualized with Cell Mask (magenta). Scale bar = 20µm. (B) Quantification of single-cell reporter intensities after 24 hours of pHi manipulation or WNT3a treatment (40 ng/mL). All cells in each field of view were quantified and were plotted if the average intensity was two-fold higher than the average of mock-transfected cells. Individual cells are shown as block dots with median and interquartile ranges shown in red lines and bars, respectively. LOW, n=103, N=5; CRL, n=181, N=7; HIGH, n=147, N=5; WNT3a, n=103, N=3. Statistical significance in (B) was determined using Kruskal-Wallis test with Dunn’s correction for multiple comparisons. *P<0.05.
    Figure Legend Snippet: (A) Representative images of MDCK cells expressing a TOPdGFP reporter plasmid shown according to scale with cell membranes visualized with Cell Mask (magenta). Scale bar = 20µm. (B) Quantification of single-cell reporter intensities after 24 hours of pHi manipulation or WNT3a treatment (40 ng/mL). All cells in each field of view were quantified and were plotted if the average intensity was two-fold higher than the average of mock-transfected cells. Individual cells are shown as block dots with median and interquartile ranges shown in red lines and bars, respectively. LOW, n=103, N=5; CRL, n=181, N=7; HIGH, n=147, N=5; WNT3a, n=103, N=3. Statistical significance in (B) was determined using Kruskal-Wallis test with Dunn’s correction for multiple comparisons. *P<0.05.

    Techniques Used: Expressing, Plasmid Preparation, Transfection, Blocking Assay



    Similar Products

    phi manipulation treatment  (Selleck Chemicals)
    96
    Selleck Chemicals phi manipulation treatment
    (A) Representative images of MDCK cells expressing a TOPdGFP reporter plasmid shown according to scale with cell membranes visualized with Cell Mask (magenta). Scale bar = 20µm. (B) Quantification of single-cell reporter intensities after 24 hours of pHi <t>manipulation</t> or WNT3a treatment (40 ng/mL). All cells in each field of view were quantified and were plotted if the average intensity was two-fold higher than the average of mock-transfected cells. Individual cells are shown as block dots with median and interquartile ranges shown in red lines and bars, respectively. LOW, n=103, N=5; CRL, n=181, N=7; HIGH, n=147, N=5; WNT3a, n=103, N=3. Statistical significance in (B) was determined using Kruskal-Wallis test with Dunn’s correction for multiple comparisons. *P<0.05.
    Phi Manipulation Treatment, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phi+manipulation+treatment/bio_rxiv__2024__03__22__586349-175-9-16?v=Selleck+Chemicals
    Average 96 stars, based on 1 article reviews
    phi manipulation treatment - by Bioz Stars, 2026-08
    96/100 stars
    		  Buy from Supplier

    Image Search Results


    (A) Representative images of MDCK cells expressing a TOPdGFP reporter plasmid shown according to scale with cell membranes visualized with Cell Mask (magenta). Scale bar = 20µm. (B) Quantification of single-cell reporter intensities after 24 hours of pHi manipulation or WNT3a treatment (40 ng/mL). All cells in each field of view were quantified and were plotted if the average intensity was two-fold higher than the average of mock-transfected cells. Individual cells are shown as block dots with median and interquartile ranges shown in red lines and bars, respectively. LOW, n=103, N=5; CRL, n=181, N=7; HIGH, n=147, N=5; WNT3a, n=103, N=3. Statistical significance in (B) was determined using Kruskal-Wallis test with Dunn’s correction for multiple comparisons. *P<0.05.

    Journal: bioRxiv

    Article Title: Intracellular pH regulates β-catenin with low pHi increasing adhesion and signaling functions

    doi: 10.1101/2024.03.22.586349

    Figure Lengend Snippet: (A) Representative images of MDCK cells expressing a TOPdGFP reporter plasmid shown according to scale with cell membranes visualized with Cell Mask (magenta). Scale bar = 20µm. (B) Quantification of single-cell reporter intensities after 24 hours of pHi manipulation or WNT3a treatment (40 ng/mL). All cells in each field of view were quantified and were plotted if the average intensity was two-fold higher than the average of mock-transfected cells. Individual cells are shown as block dots with median and interquartile ranges shown in red lines and bars, respectively. LOW, n=103, N=5; CRL, n=181, N=7; HIGH, n=147, N=5; WNT3a, n=103, N=3. Statistical significance in (B) was determined using Kruskal-Wallis test with Dunn’s correction for multiple comparisons. *P<0.05.

    Article Snippet: Where indicated, proteasome activity was inhibited 18 hours after pHi manipulation treatment using 10 μM MG132 (Selleck, S2619) for a total of 6 hours prior to sample analysis or collection.

    Techniques: Expressing, Plasmid Preparation, Transfection, Blocking Assay